Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow

Derivation of 2 categories of plasmacytoid dendritic cells in murine bone marrow.

Plasmacytoid dendritic cells (pDCs) competent to make type I interferon were rigorously defined as a Ly-6C(+) and CD11c(Lo) subset of the B220(+)CD19(-) CD43(+)CD24(Lo) bone marrow (BM) Fraction A. Otherwise similar Ly6C(-) cells expressed the natural killer (NK) markers DX5 and NK1.1. pDCs represented a stable, discrete, and long-lived population. Stem cells and early lymphoid progenitors (ELP...

Hemozoin Enhances Maturation of Murine Bone Marrow Derived Macrophages and Myeloid Dendritic Cells

Background: Falciparum malaria is a severe health burden worldwide. Antigen presenting cells are reported to be affected by erythrocytic stage of the parasite. Malarial hemozoin (HZ), a metabolite of malaria parasite, has adjuvant properties and may play a role in the induction of immune response against the parasite. Objective: To determine the immunological impact of hemozoin on the capacity ...

Bone Marrow Cultures Are Immature APCs Generated from Flt3 Ligand-Supplemented Murine Plasmacytoid Pre-Dendritic Cells

hemozoin enhances maturation of murine bone marrow derived macrophages and myeloid dendritic cells

background: falciparum malaria is a severe health burden worldwide. antigen presenting cells are reported to be affected by erythrocytic stage of the parasite. malarial hemozoin (hz), a metabolite of malaria parasite, has adjuvant properties and may play a role in the induction of immune response against the parasite. objective: to determine the immunological impact of hemozoin on the capacity ...

Study of Chondrogenic Effects of Chondrocytes Cocultured With Murine Bone Marrow-Derived Mesenchymal Stem Cells

Purpose: Co-culture systems of marrow derived mesenchymal stem cells (mMSCs) with mature chondrocytes have theoretically been considered as a putative way of MSCs chondrogenic differentiation. MSCs differentiated in this system could be used for transplantation purpose without of any need to their purification since the cells with which MSCs are co cultured are native cartilage cells. Despite o...